Characterization of lipid metabolism in a novel immortalized human hepatocyte cell 1 line . 2 Running title : Fat accumulation in a novel human liver cell line 3 4 Charlotte

Characterization of lipid metabolism in a novel immortalized human hepatocyte cell 1 line. 2 Running title: Fat accumulation in a novel human liver cell line 3 4 Charlotte J. Green, Deborah Johnson, Harsh D. Amin, Pamela Sivathondan, Michael A. 5 Silva, Lai Mun Wang, Lara Stevanato, Catriona A. McNeil, Erik A. Miljan, John D. 6 Sinden, Karl J. Morten, and Leanne Hodson 7 8 Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), University of 9 Oxford, Churchill Hospital, Oxford OX3 7LE, UK 10 2 Nuffield Department of Obstetrics & Gynaecology, The Women’s Centre, University of 11 Oxford, John Radcliffe Hospital, Headley Way, Headington, Oxford. UK 12 ReNeuron Group plc. Surrey Research Park, 10 Nugent Road, Guildford, Surrey, GU2 7AF. 13 Department of Hepatobiliary and Pancreatic Surgery, Oxford University Hospital NHS 14 Trust, Churchill Hospital, Oxford OX3 7LE, UK 15 Department of Cellular Pathology, Oxford University Hospitals, Oxford, UK 16 17 Corresponding authors: Charlotte Green and Leanne Hodson, OCDEM, Oxford University, 18 Churchill Hospital, Oxford, OX3 7LE, United Kingdom. Email: 19 [email protected], [email protected]. Phone: +44 (0) 1865 20 857225. 21 22 23 Articles in PresS. Am J Physiol Endocrinol Metab (June 30, 2015). doi:10.1152/ajpendo.00594.2014